Bio-One

Bio-One

Franzy VerifiedInformation based on 2026 FDD

Home Services · Residential Restoration

Investment min
$134.6K
Total: $134.6K–$221.1K
Avg gross revenue
$802.3K
Unit-level, 2026
Franchise fee
$60K
Per current disclosure
Royalty
7.5%
of gross revenue
Locations
144
Franchising since 2011

Description

What is Bio-One?

Biohazard and decontamination cleanup including crime scenes, hoarding, and mold remediation

Bio-One Inc. is the category-leader and first in biohazard and decontamination cleanup services growing quickly to 100+ franchise owners operating 130+ locations in 45 states. Helping people and communities in their greatest times of need, Bio-one answers the call with discretion, care, and compassion. Bio-One believes in a simple and clear mission: Help First, Business Second. A close-knit brand of community heroes devoted to cleaning up what others won’t (or can’t) including biohazard cleanup, crime scene cleanup, hoarding cleanup, mold remediation, and more. Bio-One franchisees are trained to handle these situations with compassion and professionalism, providing a valuable service to their communities. Acquired by Five Star Franchising in 2021, Bio-One is well-positioned and supported for rapid expansion and growth across North America. With low start-up costs and high profit margins, this recession-resilient industry provides an opportunity for strong leaders who are passionate about making a difference in their community.

  • Biohazard remediation
  • Trauma scene cleanup
  • Hoarding decontamination
  • Need-driven emergency service
  • B2B referral model
  • Law enforcement partnerships

Location Analysis

Where Bio-One wins

Bio-One franchises cluster around mid-to-large metropolitan areas where population density supports consistent trauma, unattended death, and hoarding call volume. Territories align with county and metro service areas rather than storefront trade zones, since work is performed mobile at client properties. Multi-unit owners commonly hold adjacent territories to consolidate crews, equipment, and on-call dispatch coverage. Presence tends to follow states with established biohazard transport and waste-handling regulatory frameworks. The model serves homeowners and residential property owners requiring remediation after death, injury, or biohazard events, along with landlords, property managers, real estate professionals, and hoarding-affected households. Institutional referral sources include law enforcement, coroners, hospitals, and insurance adjusters. Markets with high homeownership and strong property-insurance penetration may support the model, since remediation is frequently insurance-reimbursed. Candidates should validate local call volume and regulatory requirements.
Total Locations
147
Franchise units
144
Corporate locations
3
Avg. sq. footage

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Financial Analysis

The numbers behind Bio-One

Avg gross revenue$802,263
Investment range$134,645 – $221,095
Investment midpoint$177,870
Brand fund2%
Royalty7.5%
Franchise fee$60,000
Min. net worth$250,000
Min. liquid capital$60,000
Bio-One was founded in 2006 and has grown to 144 units. The initial investment ranges from $134,645 to $221,095, with a franchise fee of $60,000. Reported gross revenue is $802,263. The mobile service model requires warehouse or garage space for vehicles, personal protective equipment, chemicals, and equipment storage, along with service vehicles for crew transport. Operations demand 24/7 on-call dispatch capability due to the emergency-driven nature of biohazard remediation work. Franchisees must comply with state and local biohazard and regulated medical waste handling and transport regulations. The model does not require a retail storefront or customer-facing location, which may reduce certain overhead costs associated with traditional commercial real estate.
Did you know? Bio-One operates in the residential restoration subsector of home services. The initial investment ranges from $134,645 to $221,095. The franchise fee is $60,000. The business model is mobile, eliminating the need for a retail storefront while requiring warehouse space and service vehicles for emergency biohazard remediation work.

Financing partners

Vetted partners, tailored to franchisees

Your Franzy advisor can connect you with these partners later in the process — competitive rates, specialized in franchise financing.

FranFund

Lender

CRF USA

Nonprofit SBA lender; provides financing for franchise acquisitions, startups, and expansion.

Lender

First Bank of the Lake

Lender

Pension Pros

Lender

The model

How Bio-One works

01
Ownership
Part-Time (Executive)

Owner stays in an executive role — sets strategy, hires a manager, and oversees crews. Typically 5–20 hr/wk after ramp; many keep their day job.

Full-Time

Owner runs the business as their primary job — leads the team day-to-day on the ground, 40+ hr/wk.

02
Revenue
Revenue model not specified.
03
Customer
Customer model not specified.

FDD Item 7

Initial investment range

$134.6K–$221.1K
Most common
$134,645
Minimum
$177,870
Midpoint
$221,095
Maximum

The investment range midpoint (from range reported on Item 7) is $177,870 and financing can reduce cash-at-close by 80–90%.

FDD Item 19

Average gross sales

Avg
$528K
YOY change (2024 -> 2026)
+108%

According to Item 19 of the Franchise Disclosure Document, Bio-One has an average gross revenue of $802.3K. (Note: This information is based on the latest FDD in our records. Please review the Franchise Disclosure Document (FDD) and confirm this information directly with the brand. We make no claims of accuracy for the information presented.)

Over time

-2% YoY
144 units open as of 2026 FDD-3 in last 12 mo

2026 Franchise Disclosure

FDD documents

Below are items 2, 3, 4, 7, 11 and 19 for Bio-One's 2026 FDD. The complete FDD is delivered to you directly by the franchisor, per the FTC Franchise Rule.

Estimated initial investment
FDD Item 7 · PDF
Financial performance representations
FDD Item 19 · PDF
Members-only items
Executive team
FDD Item 2 · PDF
Litigation
FDD Item 3 · PDF
Bankruptcy
FDD Item 4 · PDF
Franchisor assistance
FDD Item 11 · PDF
Members only
Unlock the 2026 FDD

Connect to download Items 2, 3, 4, and 11 — direct from the franchisor.

Buyer FAQs

Frequently asked questions

The initial investment for a Bio-One franchise typically ranges between $134,645.00 and $221,095.00. This includes the franchise fee, equipment, real estate, and other startup costs. To get a detailed breakdown and better understand the financial requirements, we recommend scheduling a call with the Franzy team. We'll walk you through the specifics and answer any questions you might have. For more detailed information, refer to the financial sections of the FDD.

Disclaimer. The information provided on this page is based on the latest Franchise Disclosure Document (FDD) we have on record, which was issued in 2026. This information is for informational purposes only and is not intended to constitute legal, financial, or business advice. We make no guarantees or claims regarding the completeness or accuracy. Only the franchisor can confirm that the information is complete and accurate and we recommend consulting the franchisor directly for the most recent FDD and regarding any questions that you may have about the information provided.

Bio-One
Bio-One
$802.3K avg revenue · 144+ US franchises