Bio-One

Bio-One

Franzy VerifiedInformation based on 2026 FDD

Home Services · Residential Restoration

Investment min
$135K
Total: $135K–$221K
Avg gross revenue
$802K
Unit-level, 2026
Franchise fee
$60K
Per current disclosure
Royalty
7.5%
of gross revenue
Locations
144
Franchising since 2011

Description

What is Bio-One?

Bio-One Inc. is the category-leader and first in biohazard and decontamination cleanup services growing quickly to 100+ franchise owners operating 130+ locations in 45 states. Helping people and communities in their greatest times of need, Bio-one answers the call with discretion, care, and compassion. Bio-One believes in a simple and clear mission: Help First, Business Second. A close-knit brand of community heroes devoted to cleaning up what others won’t (or can’t) including biohazard cleanup, crime scene cleanup, hoarding cleanup, mold remediation, and more. Bio-One franchisees are trained to handle these situations with compassion and professionalism, providing a valuable service to their communities. Acquired by Five Star Franchising in 2021, Bio-One is well-positioned and supported for rapid expansion and growth across North America. With low start-up costs and high profit margins, this recession-resilient industry provides an opportunity for strong leaders who are passionate about making a difference in their community.

  • Biohazard remediation
  • Trauma scene cleanup
  • Hoarding decontamination
  • Need-driven emergency service
  • B2B referral model
  • Law enforcement partnerships

Location Analysis

Where Bio-One wins

Bio-One has built a footprint across a majority of U.S. states, with its origin and early concentration in Colorado, where the franchisor is headquartered. The network follows a mobile, territory-mapped structure rather than fixed storefronts, and clustering patterns show operators often expand by adding adjacent territories instead of opening new physical sites. The brand does not provide strongest regions, top states, or top markets in the input, so specific geographic concentrations beyond Colorado cannot be documented. The service model targets metro and multi-county areas with sufficient population density to sustain a flow of infrequent, incident-driven calls, though no numeric population threshold is specified. Demand originates from a broad residential base—households affected by hoarding, unattended death, suicide, homicide, or infectious contamination—as well as referral decision-makers including law enforcement, coroners, property managers, landlords, realtors, social workers, and insurance adjusters. Location requirements include no retail storefront, a home office or small commercial base, warehouse or secure storage for vehicles and equipment, fleet capacity for rapid on-site response, and compliance with bloodborne-pathogen, OSHA, and regulated medical-waste rules. Review ratings and customer sentiment data are not provided, so performance benchmarks at the territory level remain unverified. Prospective franchisees should conduct territory-level due diligence to confirm incident frequency, referral-network strength, and regulatory requirements in their target service area.
Total Locations
147
Franchise units
144
Corporate locations
3
Avg. sq. footage

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Financial Analysis

The numbers behind Bio-One

Avg gross revenue$802,263
Investment range$134,645 – $221,095
Investment midpoint$177,870
Brand fund2%
Royalty7.5%
Franchise fee$60,000
Min. net worth$250,000
Min. liquid capital$60,000
Bio-One operates in the specialized residential restoration niche of home services, with an investment range of $134,645 to $221,095 and a flat $60,000 franchise fee. This positions the concept below capital-intensive restoration formats that require brick-and-mortar facilities, reflecting a mobile, van-based service model. The system has grown to 144 units since its 2006 founding, indicating sustained franchise development over nearly two decades. Reported gross revenue of $802,263 provides a top-line benchmark, but operating margin, cost of goods sold, labor intensity, insurance expense, and regulated-waste compliance costs are not disclosed, so profitability and payback timing remain unverified. The business model is event-driven and episodic—triggered by hoarding, unattended death, biohazard contamination, and similar incidents—which may create revenue variability and require disciplined lead-generation and referral-network management. Scalability appears territory-based: operators can add adjacent service areas without opening additional storefronts, which may lower incremental expansion costs but also demands dispatch capacity and workforce readiness across wider geographies. Investor risk centers on call volume predictability, insurance reimbursement cycles, regulatory compliance burden, and the emotional complexity of the work, which can affect staffing retention and owner resilience.
Did you know? Bio-One is a residential restoration franchise in the home services industry, with a total investment between $134,645 and $221,095 and a franchise fee of $60,000. The mobile, van-based model eliminates the need for retail storefronts, focusing capital on vehicles, equipment, and compliance infrastructure.

Financing partners

Vetted partners, tailored to franchisees

Your Franzy advisor can connect you with these partners later in the process — competitive rates, specialized in franchise financing.

FranFund

Lender

CRF USA

Nonprofit SBA lender; provides financing for franchise acquisitions, startups, and expansion.

Lender

First Bank of the Lake

Lender

Pension Pros

Lender

The model

How Bio-One works

01
Ownership
Part-Time (Executive)

Owner stays in an executive role — sets strategy, hires a manager, and oversees crews. Typically 5–20 hr/wk after ramp; many keep their day job.

Full-Time

Owner runs the business as their primary job — leads the team day-to-day on the ground, 40+ hr/wk.

02
Revenue
Revenue model not specified.
03
Customer
Customer model not specified.

FDD Item 7

Initial investment range

$135K–$221K
Most common
$134,645
Minimum
$177,870
Midpoint
$221,095
Maximum

The investment range midpoint (from range reported on Item 7) is $177,870 and financing can reduce cash-at-close by 80–90%.

FDD Item 19

Average gross sales

Avg
$528K
YOY change (2024 -> 2026)
+108%

According to Item 19 of the Franchise Disclosure Document, Bio-One has an average gross revenue of $802K. (Note: This information is based on the latest FDD in our records. Please review the Franchise Disclosure Document (FDD) and confirm this information directly with the brand. We make no claims of accuracy for the information presented.)

Growth over time

Franchise footprint

-2% YoY
144 units open as of 2026 FDD-3 in last 12 mo

2026 Franchise Disclosure

FDD documents

Below are items 2, 3, 4, 7, 11 and 19 for Bio-One's 2026 FDD. The complete FDD is delivered to you directly by the franchisor, per the FTC Franchise Rule.

Estimated initial investment
FDD Item 7 · PDF
Financial performance representations
FDD Item 19 · PDF
Members-only items
Executive team
FDD Item 2 · PDF
Litigation
FDD Item 3 · PDF
Bankruptcy
FDD Item 4 · PDF
Franchisor assistance
FDD Item 11 · PDF
Members only
Unlock the 2026 FDD

Connect to download Items 2, 3, 4, and 11 — direct from the franchisor.

Buyer FAQs

Frequently asked questions

The initial investment for a Bio-One franchise typically ranges between $134,645.00 and $221,095.00. This includes the franchise fee, equipment, real estate, and other startup costs. To get a detailed breakdown and better understand the financial requirements, we recommend scheduling a call with the Franzy team. We'll walk you through the specifics and answer any questions you might have. For more detailed information, refer to the financial sections of the FDD.

Disclaimer. The information provided on this page is based on the latest Franchise Disclosure Document (FDD) we have on record, which was issued in 2026. This information is for informational purposes only and is not intended to constitute legal, financial, or business advice. We make no guarantees or claims regarding the completeness or accuracy. Only the franchisor can confirm that the information is complete and accurate and we recommend consulting the franchisor directly for the most recent FDD and regarding any questions that you may have about the information provided.

Bio-One
Bio-One
$802K avg revenue · 144+ US franchises

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